第15届国际血小板免疫学研讨会概况
第15届国际血小板免疫学研讨会于2010年6月在德国柏林召开,会议就以下4个项目(Exercise)展开讨论: (1)血小板自身抗体检测; (2)血小板HPA-1a抗体检测及抗体滴度测定; (3)针对GP IIb/IIIa (HPA-1 and HPA-3)的同种抗体检测; (4)HPA基因分型。 全球共有36家实验室参加本届研讨会,其中30家提交实验结果。中国有来自南宁、杭州、上海和广州的4家实验室参加本次研讨会,其中提交结果的有南宁输血医学研究所和浙江省血液中心两家实验室。 第15届国际血小板免疫学研讨会4个项目的研讨结果如下: (1)血小板自身抗体检测 研讨会提供2个样本(ITP1, ITP2)用于检测是否含针对GP IIb/IIIa 和GP Ib/IX的自身抗体;提供统一的MAIPA实验方法以及单克隆抗体GP IIb/IIIa (Gi5, PM6/13, SZ22),GP Ib/IX (Gi10, FMC25, MBC142.11)。结果:共有29个实验室提交该部分报告,超过19个实验室得到一致的结果。样本ITP1,22个实验室(76%)检测出含针对GPIIb/IIIa的自身抗体;样本ITP2,26个实验室(90%)检测出含针对GPIb/IX的自身抗体。 Autoantibody identification: summary of results Sample Expected result Number of participants reporting results Autoantibodies against GP … Consensus result: autoantibodies against GP …IIb/IIIa present IIb/IIIa absent Ib/IX present Ib/IX absent no conclusion ITP1 autoantibodies against GP IIb/IIIa present; against GP Ib/IX absent 29 22 5 6 21 4 IIb/IIIa present; Ib/IX absent ITP2 autoantibodies against GP Ib/IX present; against GP IIb/IIIa absent 29 14 14 26 1 3 Ib/IX present; IIb/IIIa unclear(2)血小板HPA-1a抗体检测及抗体滴度测定 Antibody identification: summary of results Sample Expected result Number of participants reporting results Reported specificitiesConsensus result HPA-1a negativeother P1 HPA-1a 28 28 0 0 HPA-1a P2 negative 28 8 20 0 negative P3 HPA-1a 28 27 0 1* HPA-1a*HPA-1a plus HPA-3b (3)针对GP IIb/IIIa (HPA-1 and HPA-3)的同种抗体检测 研讨会提供6个样本(S1, S2, S3, S4, S5, S6)供各参加实验室检测是否含针对GP IIb/IIIa (HPA-1 and HPA-3)的同种抗体,并告知可能含HPA-3抗体的疑难样本。结果:30家实验室提交了该部分报告,超过20家实验室的结果一致。 Antibody identification:summary of results Expected result Number of participants reporting results Reported specificities Consensus result HPA-1a HPA-1b HPA-3a HPA-3b mixture unknownnegative S1 negative 301 3 1 1 24 negativeS2 HPA-3a 30 26 3 1 HPA-3aS3 HPA-3a 301 61 121 negative S3* 12* 5* 1*6* none*S4 HPA-3b 30 2 1 12 1 1 13 noneS5 HPA-3b 30 1 2 2 12 2 11 noneS6 HPA-3a 30 22 6 1 1 HPA-3a*laboratories reporting on their results obtained in platelet immunofluorescence assays S1样本:正确率为77%; S2样本:所有实验室检测出S2含血小板抗体,但仅有87%实验室鉴别出含HPA-3a抗体; S3样本:含“易变” HPA-3a抗体,MAIPA法不能检出,21家实验室报“阴性”,9家实验室检测出含HPA抗体,但仅有6家((20%))检测出HPA-3a抗体,其中5/6的实验室用免疫荧光法检测; S4样本:12家实验室正确地检测出S4的HPA抗体分型,13家实验室未能发现抗体,5家实验室报告错误的抗体特异性; S5样本:12家实验室正确地检测出S5的HPA抗体分型,11家实验室未能发现抗体,7家实验室错误地报告抗体特异性; S6样本:正确率为73%,7家实验室错误地报告抗体特异性,1家实验室未能检测出抗体。 (4)HPA基因分型 研讨会提供6个DNA样本(DNA1, DNA2, DNA3, DNA4, DNA5, DNA6)用于HPA基因分型。结果:各实验室检测结果高度一致。大多数实验室(77%)6个样本HPA基因分型检测结果正确,而DNA5 和 DNA6两个样本有罕见的基因突变,影响基因分型结果(实际4家实验室受影响)。试验结果表明大多数实验室已经很好地建立了HPA基因分型检测试验方法。是否将HPA基因分型项目列入下一届研讨会。还有待讨论。 Genotyping: summary of results Lab assay* I# missing results Discrepancies DNA1 DNA2 DNA3 DNA4 DNA5 DNA6 Progenika** 6A - Brisbane 3B 4, 6 Sydney 1B 9 Vienna 1A, 1B X 6, 9 Sao Paulo 1B - Winnipeg 1A 9 Saint Laurent 1B 6, 9 HPA-1 HPA-1 Nan-Ning 1B, 5B - Hangzhou 5B - Copenhagen 1B, 5B 9 HPA-1 HPA-1 Paris 1B, 2B, 6A X - Créteil 1B, 3B - Lyon 1B 9 Berlin NR 6, 9 Rostock 1A, 1B X 6, 9 Dessau 1B, 5B X - Giessen 3B, 2B - Mannheim 1B, 5B X - Haifa 1B 6, 9 HPA-3 HPA-3 HPA-3HPA-1 HPA-3 HPA-15 HPA-3 Tokyo 6A, 1B - Kuala Lumpur 1 9 HPA-6 Tromso 1B, 3B, 6B - Warsaw 1A, 1B X - HPA-6 Ljubljana 1A, 3B X - Barcelona 1B, 6A - Geneva 1A 9 Bangkok 1 5, 9, 15 Aberdeen 1A 6, 9 HPA-1 HPA-1 Bristol 1B - HPA-15 Seattle 2B, 6A - Milwaukee 6B X -*1 = PCR-SSP; 2 = PCR-RFLP; 3 = TaqMan; 4 = LightCycler; 5 = Sequencing; 6 = other (please specify on the backside of this form); A = commercial test; B = in-house assay **Progenika participated in the genotyping exercise only #I=irregular results; indicates, whether this laboratory has observed any irregularities when typing for HPA-1 in samples DNA5 and DNA6
作者:钟周琳 校对:李恒聪 |
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