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国内首例抗HPA-5b引发的新生儿同种免疫性血小板减少性紫癜的检测、诊断及分析
(来源:南宁输血医学研究所 )
作者:周燕  钟周琳  李丽兰  申卫东  吴国光来源:中国实验血液学杂志, 2014, 22(2): 399-402.摘要  本文旨在探讨抗HPA-5b抗体引发的新生儿同种免疫性血小板减少性紫癜(NAITP)的实验检测及临床诊断。临床监测患儿血小板计数及其他临床表现;多重PCR及DNA测序检测患儿及其父母HPA-1~21bw系统基因型;流式细胞术(FCM)和血小板抗原单克隆抗体特异性免疫固定检测技术(MAIPA)检测及鉴定患儿及其母亲血清中的血小板特异性抗体。结果,患儿临床表现较轻;基因分析显示患儿为HPA-5ab,父亲为HPA-5ab,母亲为HPA-5aa;患儿母亲血清中含与父亲血小板反应的血小板特异性抗体为抗HPA-5b抗体。上述结果确诊该病例为抗HPA-5b抗体引起的新生儿同种免疫性血小板减少性紫癜。关键词:NAITP;FCM ;MAIPA;多重PCR;诊断 Detection, Diagnosis and Analysis of the First Domestic Case of Neonatal Alloimmune Thrombocytopenia purpura Associated with anti-HPA-5b in China ZHOU Yan, ZHONG Zhou-Lin, LI Li-Lan, SHEN Wei-Dong, WU Guo-GuangJournal of Experimental Hematology, 2014, 22(2): 399-402.Abstract   To study the neonatal alloimmune thrombocytopenia purpura (NAITP) caused by anti HPA-5b antibody, the platelet count and clinical manifestation in the newborn were examined. The HPA-1-21bw genotypes of the newborn and her parents were detected by multiple-PCR and DNA sequencing. The HPA-specific antibody in the sera of newborn and her mother were detected and identified by Flow Cytometry(FCM) and monoclonal antibody-specific Immobilization of Platelet Antigens (MAIPA). As a result, the clinical manifestations of the newborn were lighter. The HPA genotyping showed that the genotype of the newborn was HPA-5ab, while that of her mother and father were HPA-5aa and HPA-5ab, respectively. The antibody against the platelet of newborn’s father existed in the newborn’s mother sera. The HPA antibody of the mother was identified as anti HPA-5b. It was concluded that the newborn with neonatal alloimmune thrombocytopenia purpura was caused by the antibody against HPA-5b.Key words: NAITP; FCM ; MAIPA; Multiple-PCR; Diagnosis



时间: 2015 年 09 月 15 日
           
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