作者：周燕 钟周琳 刘金莲
来源：广西医学, 2014, 36(8):1037-1039.

摘要 目的：比较酶联免疫吸附试验（ELISA）和流式细胞术（FCM）检测血小板自身抗体的特点，探讨其对特发性血小板减少性紫癜（ITP）的诊断意义。方法：42例ITP患者，分别采用FCM和ELISA检测血小板表面及血清中的抗血小板自身抗体。 结果：FCM检测到血小板自身抗体阳性36例，阳性率为85.7%；ELISA检测到血小板自身抗体阳性30例，阳性率为71.4%，两种方法检出率无统计学差异（P>0.05），但是FCM和ELISA方法检测血小板表面抗体阳性率均高于血清（P均<0.05）。结论：FCM和ELISA检测结果均准确可靠，且各具特征。FCM具有快速、方便的特点更适合初筛使用，与ELISA合用可进一步提高ITP患者的免疫诊断水平。
关键词：血小板减少性紫癜；血小板自身抗体；酶联免疫吸附实验；流式细胞术；

Enzyme-Linked Immunosorbent Assay versus Flow Cytometry in Detection of Platelet Autoantibody: A Comparative Analysis

ZHOU Yan, ZHONG Zhou-lin, LIU Jin-lian
Guangxi Medical Journal, 2014, 36(8):1037-1039.

[Abstract] Objective To compare the features of enzyme linked immunosorbent assay(ELISA) and Flow Cytometry(FCM) in platelet autoantibodies detecting, and study the diagnostic significance of these assays in ITP patients. Methods A total of 42 ITP patients are detected platelet autoantibodies both in platelet member and seurm by ELISA and FCM assays ,respectively. Results A total of 36 ITP patients are found platelet autoantibodies by FCM, compare 30 by ELISA. The positive rate of platelet autoantibody by FCM is 85.7%, while that of ELISA is 71.4%. There is no significant difference between two methods（P>0.05）. The positive rate of platelet autoantibody from platelet member are higher than that from serum in both FCM and ELISA assays(P<0.05). Conclusion The two methods with their features are reliable and exact. FCM assay is a rapid and sensitive method for screening, and associate with ELISA would improve the immunodiagnosis level of ITP patients. 
Key words Idiopathic thrombocytopenic purpura; platelet autoantibody; Enzyme-Linked immunosorbent assay; Flow cytometry;