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酶联免疫吸附试验与流式细胞术检测血小板自身抗体的比较
(来源:南宁输血医学研究所 )

作者:周燕 钟周琳 刘金莲
来源:广西医学, 2014, 36(8):1037-1039.

摘要 目的:比较酶联免疫吸附试验(ELISA)和流式细胞术(FCM)检测血小板自身抗体的特点,探讨其对特发性血小板减少性紫癜(ITP)的诊断意义。方法:42例ITP患者,分别采用FCM和ELISA检测血小板表面及血清中的抗血小板自身抗体。 结果:FCM检测到血小板自身抗体阳性36例,阳性率为85.7%;ELISA检测到血小板自身抗体阳性30例,阳性率为71.4%,两种方法检出率无统计学差异(P>0.05),但是FCM和ELISA方法检测血小板表面抗体阳性率均高于血清(P均<0.05)。结论:FCM和ELISA检测结果均准确可靠,且各具特征。FCM具有快速、方便的特点更适合初筛使用,与ELISA合用可进一步提高ITP患者的免疫诊断水平。
关键词:血小板减少性紫癜;血小板自身抗体;酶联免疫吸附实验;流式细胞术;


Enzyme-Linked Immunosorbent Assay versus Flow Cytometry in Detection of Platelet Autoantibody: A Comparative Analysis

ZHOU Yan, ZHONG Zhou-lin, LIU Jin-lian
Guangxi Medical Journal, 2014, 36(8):1037-1039.

[Abstract] Objective To compare the features of enzyme linked immunosorbent assay(ELISA) and Flow Cytometry(FCM) in platelet autoantibodies detecting, and study the diagnostic significance of these assays in ITP patients. Methods A total of 42 ITP patients are detected platelet autoantibodies both in platelet member and seurm by ELISA and FCM assays ,respectively. Results A total of 36 ITP patients are found platelet autoantibodies by FCM, compare 30 by ELISA. The positive rate of platelet autoantibody by FCM is 85.7%, while that of ELISA is 71.4%. There is no significant difference between two methods(P>0.05). The positive rate of platelet autoantibody from platelet member are higher than that from serum in both FCM and ELISA assays(P<0.05). Conclusion The two methods with their features are reliable and exact. FCM assay is a rapid and sensitive method for screening, and associate with ELISA would improve the immunodiagnosis level of ITP patients. 
Key words Idiopathic thrombocytopenic purpura; platelet autoantibody; Enzyme-Linked immunosorbent assay; Flow cytometry;


时间: 2015 年 09 月 15 日
           
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